Fig. 4: Indistinct lineage-specific H3K9me3 in SCNT embryos during the first cell fate determination. | Nature Communications

Fig. 4: Indistinct lineage-specific H3K9me3 in SCNT embryos during the first cell fate determination.

From: Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos

Fig. 4

a PCA of H3K9me3 signals in the ICM and TE of fertilized and SCNT embryos. b H3K9me3 signals in the ICM and TE of fertilized and SCNT embryos; the regions with the most positive and negative loading (n = 3427, Supplementary Fig. 4b) were classified into 7 clusters based on H3K9me3 signals using the k-means function. c H3K9me3 signals in the morula of fertilized and SCNT embryos, with these regions corresponding to (b). d The differences in H3K9me3 levels at TF binding sites between fertilized and SCNT embryos; left panel: ICM, right panel: TE. The Y-axis shows the −log10 transformed q-values calculated using Fisher’s exact test, and the X-axis shows the log2-fold change values. The red plots represent the potential TFs that may be related to defective differential H3K9me3 deposition in SCNT blastocysts. The blue plots represent well-known chromatin architecture-related and H3K9me3-related factors. The gray plots represent other TFs. Source data are provided as a Source Data file.

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