Fig. 8: Exosomal miR-204 is associated with leptin signialing in BC patients. | Nature Communications

Fig. 8: Exosomal miR-204 is associated with leptin signialing in BC patients.

From: Cancer-cell-secreted miR-204-5p induces leptin signalling pathway in white adipose tissue to promote cancer-associated cachexia

Fig. 8

The qRT-PCR analysis of miR-204 abundance in various breast cancer cells (n =  3 biologically independent samples per group; unpaired two-tailed t-test) (a) and sEVs fraction (n = 3 biologically independent samples per group; unpaired two-tailed t-test). b The qRT-PCR analysis of miR-204 abundance in several cancer cell lines and their derived sEV fractions: (c) C26 and HCT116 colon cancer (n = 3 biologically independent samples per group; one-way ANOVA followed by Dunnett multiple comparison test), (d) LLC, A549 lung cancer cells (n = 3 biologically independent samples per group; one-way ANOVA followed by Dunnett multiple comparison test) and (e) MGC-803 gastric cancer cells (n = 3 biologically independent samples per group; unpaired two-tailed t-test). f Western blots showing indicated proteins in differentiated primary adipocytes treated various breast cancer sEVs. Repeated three times independently with similar results obtained. g Relative mRNA abundance of the indicated genes in human stromal vascular fraction (SVF) treated indicated sEVs (n = 3 biologically independent samples per group; one-way ANOVA followed by Dunnett multiple comparison test) and (h) the related protein levels. i The qRT-PCR determined miR-204 levels in normal donor serum sEVs and breast cancer patient’s serum sEVs (n = 6 per group; unpaired two-tailed t-test). j The qRT-PCR determined miR-204 levels in tumour tissue and sEVs (n = 3 biologically independent samples per group). k Serum leptin level in nomal donor and breast cancer patient (n = 18 per group). l The qRT-PCR determined miR-204 levels in BC patients with obesity and BC cachexia patient’s serum sEVs (n = 6 per group; unpaired two-tailed t-test). m Western blots showing indicated proteins in control WAT from non-cancer patients or breast cancer patient’s WAT. Repeated three times independently with similar results obtained. n Representative ISH images and immunohistochemistry staining of HIF1Α, UCP1 and LEPTIN in tumour associated adipose tissues. Repeated three times independently with similar results obtained. Scale bar, 200 μm. o Schematic model was created using BioRender (https://biorender.com/) and Adobe Illustrator. Data are presented as mean±s.e.m; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: not significant. Source data and exact P value are provided as a Source data file.

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