Fig. 5: Using CAST based ASOs to regulate cellular gene expression. | Nature Communications

Fig. 5: Using CAST based ASOs to regulate cellular gene expression.

From: Programming conformational cooperativity to regulate allosteric protein-oligonucleotide signal transduction

Fig. 5

a Schematic illustration of the allosteric regulation of GFP gene expression via thrombin. b The whole processes of the experimental setup in vivo. c Confocal microscopy imaging results. Scale bars: 200 μm. Schematic illustration (d) and the confocal microscopy images (e) of CAST regulation of GFP gene expression using two regulators T1 and T2, respectively. Scale bars: 200 μm. Relative GFP-positive cells (f) and mean fluorescence intensities (g) of CAST-regulated GFP gene expression, respectively. f, g Data collected in e were quantified using ImageJ software and are presented as mean ± s.d. for n = 4 biologically independent experiments. Source data are provided as a Source Data file. Statistic analysis for f and g was performed using two-sided test (*p ≦ 0.05, **p ≦ 0.01, ***p ≦ 0.001).

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