Fig. 3: Reversible transitions in both the lamellipodium structure and intracellular dynamics take place when regulating cell migration speed. | Nature Communications

Fig. 3: Reversible transitions in both the lamellipodium structure and intracellular dynamics take place when regulating cell migration speed.

From: Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion

Fig. 3

a The CMFDA images of a live cell. For the cell in the slow mode with a fluorescence gap in the lamellipodium (left panel), 25 nM Calyculin A is added to enhance the myosin II activity. The cell speed then increases, and the gap disappears accordingly (right panel). Scale bar, 10 μm. b Intensity profiles of CMFDA along the white line across the lamellipodium from the cell body to its leading edge, as shown in (d). c Comparison of cell speed of the same cell before and after the calyculin A treatment (n = 7). d The lamellipodium reversibly changes from the gap state to the flat shape when the cell separates from other cells and loses mechanical loading. e The intensity profile of CMFDA along the white line from the cell body to the leading edge shows the dynamical changes of lamellipodium fluorescence, which indicates the lamellipodium thickness. In the beginning, the CMFDA intensity at the rear lamellipodium is obviously lower than that at the front part. After 195 s, when the cell is separated from the population, the intensity difference between the rear and front lamellipodia is eliminated. f, g Diffusion maps (f), and projection of trajectories (g) of QDs for the same cell before and after the treatment with 1% PEG. h Average D and migration speeds for cells before and after PEG treatment (fast, n = 6; PEG, n = 6). Data are shown as mean ± SD. Source data are provided as a Source Data file.

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