Fig. 3: Emergence of the unique CR ensemble after fear conditioning. | Nature Communications

Fig. 3: Emergence of the unique CR ensemble after fear conditioning.

From: Activity-dependent organization of prefrontal hub-networks for associative learning and signal transformation

Fig. 3

a Schematic diagram for extracting the RS ensemble (RSE) with building the RS model. As a neural activity, z-normalized ΔF/F is shown. b–d Estimating and decoding locomotion states by the RS model. b In an example mouse, the RS model possessed high performance for estimating RS (day[D]4-interval; top) and decoding locomotion states during CS+ at D3-early (D3E; middle). However, the performance decreased for the locomotion states during CS+ at D4-early (D4E; bottom). c No significant difference in the original RS-model performance between D3 and D4. d (left) Significant decrease in the RS-model decoding performance for locomotion states during CS+ at D4E, compared with D3E. (right) The changes in the decoding performance visualized by subtracting D3E values from others (D3-late [D3L], D4E, D4-late [D4L])). e Schematic diagram for comparing the overlap between the CR ensemble (CRE) and the RSE. f A Venn diagram and a spatial map of an example mouse showing the limited overlap between the CRE and RSE. g Summary of the overlap between the CRE and RSE of all 7 mice (n = 1165 neurons). h The decoding performance of the CR model to the RS was statistically compared with the fitting performance of the CR. Of 11 successfully imaged mice, 7 mice were longitudinally imaged on D3-D4. Because data from 1 of the 7 mice on D3 did not meet the RS-modeling criteria, N = 10 for D3 (c, d); N = 6 for D3-D4 paired comparison (d); N = 7 for D4 (c and Supplementary Fig. 8). The CR models (at D4E) were successfully built in all seven mice (h). The fitting/decoding performances are indicated by the accuracy, while the AUC was similar (Supplementary Fig. 8). Two-tailed, Wilcoxon rank-sum test (c, p = 0.887) and paired permutation tests (d-left, p = 0.016; d-right, p = 0.244, 0.016, 0.531 from left to right; h, p = 0.008) were used. *p < 0.05; **p < 0.01; n.s., not significant. Red bars, median; box in d-right, 25th–75th percentiles. Source data are provided as a Source Data file.

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