Fig. 1: I. orientalis as an industrial platform for succinic acid production. | Nature Communications

Fig. 1: I. orientalis as an industrial platform for succinic acid production.

From: An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis

Fig. 1

A Our engineered I. orientalis strain can produce succinic acid at low pH, eliminating the requirements of base and acidification and lowering the overall production cost. The ‘Sugarcane’ illustration was created by Mirhee Lee, courtesy of Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign. B A schematic diagram for succinic acid production by engineered I. orientalis strain. Deleted genes are marked with delta (∆) symbol. Overexpressed genes are marked in red. G6P glucose 6-phosphate, GADP glyceraldehyde 3-phosphate, 1,3-BPG 1,3-bisphosphoglycerate, DHAP dihydroxyacetone phosphate, DHA dihydroxyacetone, G3P glycerol 3-phosphate, OAA oxaloacetate, HXK hexokinase, NDE external NADH dehydrogenase, GPD glycerol-3-phosphate dehydrogenase, PDC pyruvate decarboxylase, PYC pyruvate carboxylase, MDH malate dehydrogenase, FUMR fumarase, FRD fumarate reductase, MAE1 dicarboxylic acid transporter, SUC2 invertase, GDH glycerol dehydrogenase, DAK dihydroxyacetone kinase, g3473 dicarboxylic acid importer.

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