Fig. 4: Comparison between anti-CTLA4 and anti-PD-1 monotherapy treated mice showed shared and distinct patterns of mucosal immune activation.

a Venn diagram of the DEGs (FDR < 0.05) identified by comparing the expression profiles of whole colon biopsies from wildtype female 6-week-old mice with only anti-CTLA4 treatment (n = 3) or anti-PD-1 treatment (n = 3) in comparison to control wildtype female 6-week-old mice (n = 3). b Chair plots showing differentially expressed transcripts ranked by decreasing log fold change and coloured by estimated FDR in wildtype female 6-week-old mice with only anti-CTLA4 treatment (n = 3) or anti-PD-1 treatment (n = 3) in comparison to control wildtype female 6-week-old mice (n = 3). c Circular plot showing activation score of biological pathways which were significantly impacted, using IPA Canonical Pathways, in wildtype female 6-week-old mice with only anti-CTLA4 treatment (n = 3) or anti-PD-1 treatment (n = 3) in comparison to control wildtype female 6-week-old mice (n = 3). d Hallmark pathway analysis using GSEA in the gene expression changes in the distal colon of wildtype female 6-week-old mice with only anti-CTLA4 treatment (n = 3) or anti-PD-1 treatment (n = 3) in comparison to control wildtype female 6-week-old mice (n = 3). P value adjustment for multiple comparisons was applied with the Benjamini and Hochberg method. e Radar plot showing log2 fold change for the top 20 genes associated to the IFNγ responsive pathway in the gene expression changes in the distal colon of wildtype female 6-week-old mice with only anti-CTLA4 treatment (n = 3), anti-PD-1 treatment (n = 3) or combination treatment (n = 3), in comparison to control wildtype female 6-week-old mice (n = 3). f GSEA running score for the IFNγ responsive pathway in the gene expression changes in the distal colon of wildtype female 6-week-old mice with only anti-CTLA4 treatment (n = 3), anti-PD-1 treatment (n = 3) or combination treatment (n = 3), in comparison to control wildtype female 6-week-old mice (n = 3).