Fig. 6: CPI-induced colitis exhibits a profound and increased cytotoxic phenotype.

a UMAP plot and b the Pearson correlation showing the increased occurrence of Ifng and Gzmb expressing cells in CPI-induced colitis (n = 3) vs control samples (n = 4). P value adjustment for multiple comparisons was applied with the Benjamini and Hochberg method. c Heatmap of cytokine and chemokine expression shown by log₂ fold changes between CPI-induced colitis (n = 3) and control samples (n = 4) in colonic CD4⁺ and CD8⁺ T cell clusters. d Representative flow cytometry histograms and bubble plots showing the percentage of granzyme B, IFNγ and perforin expressing CD3⁺ CD4⁺ T cells and e CD3⁺ CD8⁺ T cells in wildtype female 6-week-old mice with CPI-induced colitis (n = 16) and control wildtype female 6-week-old mice (n = 16). f Concentrations of IFNγ and granzyme B were determined via Luminex of supernatants from cultured colonic lamina propria cells at 2 × 10⁶/ml for 24 h with plate bound anti-CD3 (2 µg/ml) for untreated Balb/C wildtype female 6-week-old mice (Control: n = 4) and wildtype female 6-week-old mice treated with CPI colitis model (CPI + FMT: n = 4). Mean and standard deviation are shown. *P < 0.05 two-sided Mann–Whitney U T test performed. g Colon mass (Control: n = 21) or wildtype female 6-week-old CPI colitis mice treated with an isotype control (CPI colitis: n = 21) or anti-IFNγ (Anti-IFNγ: n = 24), Kruskal-Wallis Test with Dunn’s multiple corrections performed where **P = 0.0014 and ****P < 0.0001. h Average colitis score, using the same previous colitis score parameters (Control: n = 7) or wildtype female 6-week-old CPI colitis mice treated with an isotype control (CPI colitis: n = 17) or anti-IFNγ (Anti-IFNγ: n = 8). Bar graph showing mean with SEM and Two-way ANOVA with Tukey’s multiple comparison test used whereby ***P = 0.0003 and ****P < 0.0001. i Number of Gr-1^hi neutrophils of untreated wildtype female 6-week-old Balb/C mice (Control: n = 25) or wildtype female 6-week-old CPI colitis mice treated with an isotype control (CPI colitis: n = 43) or anti-IFNγ (Anti-IFNγ: n = 24) Kruskal–Wallis Test with Dunn’s multiple corrections performed where **P = 0.0025 and ****P < 0.0001.