Fig. 1: Hepatic FGF18 is upregulated in HSCs after hepatic I/R injury.

A RNA sequence was carried out between I/R and Sham. The results of FGFs changes are presented in the form of heat maps (n = 3). B The protein expression of FGF18 in human subjected to hepatic resection (n = 4). C The protein expression of FGF18 in livers of mice subjected to I/R (1 h/6 h) surgery or not (n = 3). Non-ischemia represented non-ischemia liver lobes and ischemia represented ischemia liver lobes. D The ratio of FGF18/GAPDH after hepatectomy positively correlated positive with ALT at POD1 (n = 32). E The serum level of FGF18 in human subjected to hepatic resection (n = 5). F The protein expression of FGF18 in different tissues under hepatic I/R (1 h/6 h) or not (n = 3). G KCs, LSECs, HSCs, and hepatocytes were extracted from mouse liver and subjected to H/R (4 h/6 h) challenge. All the primary liver cells were verified for purity by immunofluorescence. Scale bar = 200 μm. Then cells were carried out for RT-PCR assay (n = 3). (Scheme is Created with BioRender.com) H Immunofluorescence for FGF18 (red) and different cell markers (green). Scale bar = 50 μm (n = 5). I/R ischemia reperfusion, HSCs hepatic stellate cells, KC Kupffer cell, LSEC liver sinusoids endothelial cell; The statistical significance of differences were assessed by two-tailed student unpaired t-test for (B, C, E). Other assays were assessed by one-way ANOVA, followed by Tukey’s multiple comparison test. Data are presented as means ± SEM with individual values. All numbers (n) are biologically independent experiments. Source data are provided as a Source Data file.