Fig. 7: Characterization of Lr47.

a Transcript levels of Lr47 in mock-inoculated and Pt-inoculated plants. Leaves were collected at five time points: 0 h, 1 dpi, 2 dpi, 4 dpi, and 6 dpi. Transcript levels were determined in at least seven biological replicates (n ≥ 7) and expressed as fold-Actin. Gray open dots represent single data points. The significance of the differences was estimated using two-sided unpaired t-test. Error bars indicate SEMs. ns = not significant. b Schematic diagram of conserved domains in the Lr47 protein. NLS, the predicted nuclear localization signal. c Subcellular localization of the GFP-fused Lr47 protein in tobacco leaves. This experiment was repeated three times with consistent results. Scale bars represent 50 or 100 μm. BF, bright field; GFP, green fluorescent protein. d GFP-horseradish peroxidase Western blot showing proteins expressed at expected sizes for all constructs. Target bands are highlighted by blue arrowheads. 1, empty vector; 2, GFP; 3, GFP-Lr47_CC; 4, Lr47_CC-GFP; 5, Lr47_CDS-GFP; 6, GFP-Lr47_CDS. The experiment was repeated twice with consistent results. e Macroscopic cell death in N. benthamiana leaves 48 hpi with A. tumefaciens carrying Lr47 constructs. No cell death was observed in leaf regions transiently overexpressing Lr47 and its protein domains individually. CC coiled-coil, NB nucleotide binding, LRR leucine-rich repeat, CDS the coding regions of Lr47, BAX a mammalian cell death inducer as positive control. Source data are provided as a Source data file.