Fig. 3: Neutrophils in aged mice exhibit altered chemotactic gene expression and tissue localization.
From: A spatial sequencing atlas of age-induced changes in the lung during influenza infection
A Dot plot showing predicted interactions between CXC chemokine ligands produced by cells labeled on the x-axis and CXC chemokine receptors located on neutrophils at day 9 post-infection. Color denotes communication probability, size denotes p-value. B, C Representative flow plots B and frequencies C of CD11b+ Ly6G+ neutrophils on day 9 post-infection in young and aged lungs. D Visium plots showing locations of vascular and parenchymal regions of lung based on vascular gene expression. E Violin plots comparing neutrophil localization in vascular and parenchymal regions between young and aged mice, based on (D). Statistical testing was performed using a two-sided Wilcoxon test without multiple comparison correction (A–E) or two-sided two-sample t-test (C). Error bars in (C) denote mean ± standard deviation. Flow cytometry data are pooled from two independent experiments, n = 3 mice per age group per experiment. Source data are provided as a Source Data file.
