Fig. 1: Single-cell analysis of mutant CALR protein expression.
From: Proteogenetic drug response profiling elucidates targetable vulnerabilities of myelofibrosis
A Staining of a cell line panel with the CALR mutant-specific antibody CALRm. Representative images of the included cell lines are shown (left panels; blue: DAPI; red: CALRm). Boxplots (right) represent single-cell CALRm intensities for the panel as quantified by intracellular flow cytometry analysis (right panel). p-Values indicate Student’s t-test significance: **** = p < 0.0001. Exact p-values are reported in Source Data. Box plots indicate the median (horizontal line) and 25% and 75% ranges (box), and whiskers indicate the 1.5× interquartile range above or below the box. B Single-cell immunofluorescence imaging of MF PBMCs stained with CALRm. Representative images of different MF PBMC samples are shown (left panels; blue: DAPI; red: CALRm). Single-cell mean CALRm intensities of four replicate wells with 25 images per well are shown as a violin plot with percentages of thresholded CALRm-positive cells annotated as text (right panel). Representative results of two independent repeats are shown. Violin colors indicate the CALR VAF of the corresponding MF patient. See also Supplementary Fig. 1.
