Fig. 5: A palmitic acid-enriched diet increases, but PHF2 decreases mice tumor growth.
a Schematic diagram of the in vivo model. Hep3B cells were transfected with luciferase-IRES-GFP-pcDNA (Vector), luciferase-IRES-GFP-WT-PHF2, or luciferase-IRES-GFP-PHF2-C23A. The cells from a single colony (5 × 105) by G418 selection were transplanted by orthotopic injection into the livers of the immunodeficient NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice. Mice were fed a PA-enriched diet (PAD) or normal diet (ND) for 28 days (n = 7 independent animals in each group). b Bioluminescence images of mice obtained every 7 days after transplantation using the Xenogen IVIS® Lumina Spectrum. Color scale bars represent luminescence intensity ranging from low (purple) to high (red). c Total flux (photons/s/cm2/sr) was measured and growth curves were plotted based on the bioluminescence intensities. Mean ± SD (n = 7 independent animals in each group); *P < 0.05. d (Top) Representative images of liver morphology. (Bottom) Hematoxylin and Eosin (H&E) staining and immunohistochemical analysis of tumor sections using the indicated antibodies and DAB staining. The scale bar represents 400, 100, and 25 µm in images taken at ×4, ×100, and ×400 magnifications, respectively. e The body weight of mice was measured at the indicated time point; mean ± SD (n = 7 independent animals for each group); *P < 0.05. f Total FFAs levels in mice serum and tumor tissues were presented; mean ± SD (n = 3 independent samples); *P < 0.05. g The expression levels of the indicated proteins in tumors of mice were analyzed using western blotting. n = 3 independent experiments. h mRNA levels of lipogenesis- and proliferation-related genes in tumors were quantified relative to 18S mRNA; mean ± SD (n = 3 independent experiments); *P < 0.05. For the analyses in (c, e, f, h), statistical significance was evaluated by a two-tailed Student’s t test. The exact p values are presented in Supplementary Data 2. Source data are provided as a Source Data file.
