Fig. 4: Two structures of the KCNQ2-CaM F104A mutant determined from one dataset in the presence of CBD and PIP2. | Nature Communications

Fig. 4: Two structures of the KCNQ2-CaM F104A mutant determined from one dataset in the presence of CBD and PIP2.

From: Ligand activation mechanisms of human KCNQ2 channel

Fig. 4

a Two different KCNQ2-CaMF104A maps were reconstructed from one dataset for KCNQ2 F104A mutant with both CBD and PIP2. b The density maps of one CBD molecule in different orientations at the contour level of 3.7 σ. c The density maps of one CBD molecule and one PIP2 molecule in different orientations at the contour level of 3.7 σ and 3.5 σ, respectively. d The closed activation gate of KCNQ2-CaMF104A-CBD-PIP2(-)-I. The dashed lines show diagonal atom-to-atom distance (in Å) at the constriction-lining residues Gly310, Ser314, and Leu318. e The open activation gate of KCNQ2-CaMF104A-CBD-PIP2-II. The dashed lines show diagonal atom-to-atom distance (in Å) at the constriction-lining residues Gly310, Ser314, and Leu318.

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