Table 1 Raman peak assignment

1160-1170

DNA^42,47

1230

Antisymmetric phosphate stretching⁴⁷

1375

T, A, G ring breathing modes^42,47

1490-1515

DNA & C⁴⁷

1574

G, A^42,47

1601

Thymine⁴²

Cy3.5 Peaks (cm⁻¹)

Assignment

1270-1280

Motion of aromatic groups^33,34

1350

Methine chain motion^33,34,35

1463

CH₃ asymmetric deformation^33,34,35

1560 & 1590

N⁺=C stretching motion^33,34,36

1610 − 1620

C=C stretching mode^34,36

Biotin Peaks (cm⁻¹)

Assignment

1270

Methylene group wagging^43,44

1470

Stretching of ring C-H₂^43,44

1565

C-N stretching^43,45

Streptavidin Peaks (cm⁻¹)

Assignment

1239

Amide III/β-sheet^43,44,46,47

1336

Tryptophan W7^43,44,46

1367

Tryptophan W7^43,44,46, also DNA ring breathing^42,47

1560-1580

Tryptophan W2^43,46

1670

Amide I/β-sheet^43,46,47

Thrombin Peaks (cm⁻¹)

Assignment

1230-1250

Amide III/β-sheet^47,50

1302−1310

Amide III or CH₂ twisting in proteins or A, C⁴⁷

1360

Tryptophan^47,50

≈1550−1560

Amide II or tryptophan^47,50

1639−1670

Amide I/β-sheet^47,50,51