Fig. 3: The differentiation of trNK is denoted by 4-1BB and CD55 expression.

a RNA velocity of the differentiation trajectory of uterine trNK. b Pseudotime inference of uterine trNK differentiation in a two-dimensional state-space defined by Monocle2 (left), and ordering of each uterine trNK subgroup along the trajectory (right). c Distribution of each uterine trNK subgroup along the pseudotime trajectory. d Gene expression dynamics of uterine trNK along pseudotime trajectory. e Expression of the indicated effector genes in uterine trNK, respected to pseudotime coordinate. f Expression of the indicated transcription factors in uterine trNK, respected to pseudotime coordinate. g Expression of the indicated surface molecules in uterine trNK, respected to pseudotime coordinate. h Flow cytometry showing co-expression of 4-1BB and CD55 on uterine trNK in virgin and gd8.5 mice. i Giemsa-Wright staining showing the morphology of uterine trNK-V, trNK-D and trNK-T subgroups from gd8.5 dams. j Deconvolution of the transcriptomes (bulk RNA-Seq) of uterine trNK-V, trNK-D and trNK-T subgroups, using the gene signatures of virginal, differentiating, and terminal trNK subgroups (based on scRNA-Seq). Numbers indicate the percentages in each box. Data are shown as the mean ± SEM. Data are representative of two independent experiments (a–j) or at least three independent experiments (h–i). Source data are provided as a Source Data file.