Fig. 4: Detection of NlToEVE14 protein and its expression profiles in different tissues and developmental stages of Nilaparvata lugens.

a Peptide of NlToEVE14 identified with proteomic analysis. b Efficient knockdown of NlToEVE14 transcript with dsNlToEVE14 injection (dsGFP was used as the control). P-values were determined by two-tailed unpaired Student’s t test. ***P < 0.001. c The presence of NlToEVE14 protein was confirmed by Western blotting with a specific antibody. The band with the expected size of NlToEVE14 (~100 kDa) is indicated with a red arrow, whereas the non-specific band (NB, ~70 kDa) is marked with a black arrow. The ~100 kDa band became weaker after dsNlToEVE14 treatment, while the ~70 kDa band was not significantly affected. d Expression profiles of NlToEVE14 transcripts in the eggs, nymphs (1^st −5^th in^stars), and adults (female and male) of N. lugens. e Protein expression profiles of NlToEVE14 in the 4^th and 5^th instar nymphs of N. lugens. Transcript (f) and protein (g) expression profiles of NlToEVE14 in different tissues of N. lugens. SG Salivary gland, FB Fat body, Car Carcass, WiB Wing buds. Data in b, d, and f are presented as mean values ± SEM (n = 3 independent biological replicates). The experiments in c, e, and g were repeated three times with similar results. Source data are provided as a Source Data file.