Fig. 1: Structure of hSMPD2.
From: Molecular basis for the catalytic mechanism of human neutral sphingomyelinases 1 (hSMPD2)
a Hydrolysis of sphingomyelin by sphingomyelinases. b Relative enzyme activity of wild type hSMPD2 and hSMPD3 measured using the Amplex Red Sphingomyelinase Assay Kit. Data shown are mean +/− SEM (n = 4, biological replicates). Source data are provided as a Source Data file. c Cryo-EM map of the high-resolution hSMPD2 structure from three different views. The two protomers are indicated in orange and purple. d Ribbon representation of hSMPD2 from a side view. The two protomers are indicated in orange and purple. e Cylindrical representation of a hSMPD2 protomer. Different regions are indicated with different colors. f Schematic representation of the secondary structure of hSMPD2.
