Fig. 2: Identification of DMRs between monkey ICSI and SCNT blastocysts.

a Schematic diagram illustrating the production of hybrid ICSI and SCNT embryos. The abbreviation of M.f. indicates Macaca fascicularis, while M.m. is the short for Macaca mulatta. b Scatterplot comparing the DNA methylation levels between each sample. Pairwise comparison of the average DNA methylation levels in 10 kb windows. White represents regions with the lowest methylation, while red represents regions with the highest methylation. c The boxplot compares DNA methylation levels between monkey ICSI and SCNT blastocysts. Pairwise comparisons of average DNA methylation levels within 10 kb windows (n = 292,059). The upper and lower edges of the box represent the third and first quantiles, respectively, while the thick lines in the middle indicate the medians of each sample. The statistical analysis employed a two-sided Wilcoxon rank-sum test without adjustment, resulting a p-value of <2.2e-16 (***). The red dots on the plot represent the means of each sample type. Two replicates were included for each sample (n = 2). ICSI, monkey ICSI blastocysts. SCNT, monkey SCNT blastocysts. d Boxplots displaying the DNA methylation levels of ICSI and SCNT blastocysts at hyperDMRs (n = 9013) and hypoDMRs (n = 10,076). The upper and lower edges of the box indicate the third and first quantiles, respectively. The thick lines in the boxes indicate the medians, and the red dots represents the means for each sample type. The number of DMRs is indicated in the figure. e The boxplot compares the lengths of hyperDMRs and hypoDMRs. The upper and lower edges of the box indicated the third and first quantiles, respectively, while the thick lines in the middle indicate the medians of hyper- and hypoDMRs. The red dots represent the means for each sample type.