Fig. 2: Different patterns of transcriptome heterogeneity within cell lines.
From: Single cell multi-omics reveal intra-cell-line heterogeneity across human cancer cell lines
a Illustration of two types of transcriptional heterogeneity: continuous vs discrete; UMAP plot showing exemplary cell lines for different patterns: Hs 578T for discrete and A549 for continuous. b Diversity scores for different cell lines, cell lines with light blue indicating continuous pattern and cell lines with dark blue indicating discrete pattern. A violin plot depicted the diversity score of the cell lines with the two patterns, including continuous (n = 18 cell lines) and discrete (n = 24 cell lines). For each boxplot, the center line represents the median, the box indicates the upper and lower quartiles and the whisker represents 1.5-fold of the interquartile range. A one-sided Wilcoxon test was used to test the statistical significance (p-value = 0.021). c The main heatmap depicts pairwise similarities between all NMF programs, quantified by Jaccard Index over the programs’ genes, ordered by hierarchical clustering. Twelve clusters are indicated by squares and numbers. d Annotation and selected top marker genes were shown for each cluster. Source data are provided in the Source Data file.
