Fig. 6: Enhancing root adaptation of LN through H3K27me3 rewiring.

a Dynamic H3K27me3 peaks in “BobWhite” (BW) and the Taswn-cr mutant plants under NN and LN conditions. b Proportion of TaSWN-dependent up-regulated H3K27me3 peaks induced by LN/NN conditions in KN9204 and J411. The numbers indicate total number of LN-induced H3K27me3 peaks. c Overlap between genes marked by TaSWN-dependent H3K27me3 and genes marked by LN-induced H3K27me3 in J411. Significance assessed using one-sided Fisher’s exact test for overlaps. d Dynamic changes of H3K27me3 level influenced by TaSWN under different nitrogen conditions (NN and LN) in KN9204, J411, BW, and Taswn-cr plants. e GO enrichment of genes within different categories mentioned in (d) (two-sided Fisher’s exact test, BH for multiple comparisons). f Expression changes and fold-changes of genes within different categories mentioned in (d) (two-sided Wilcoxon test). FC: fold-change. Boxplots include a median with quartiles and outliers above the top whisker. g Scans of root systems of BW and Taswn-cr seedlings grown under different nitrogen conditions. Experiment repeated two times with similar results. Scale bars = 5 cm. h The number of root tips and total length of the root systems for BW and Taswn-cr seedlings grown under different nitrogen conditions (two-sided Student’s t-test). Data are shown as mean ± s.d. of five biological replicates. i Transcription fold-changes of NRT2 genes under LN conditions in BW, Taswn-cr, J411, and KN9204 roots. j ¹⁵N-nitrate uptake activity of different seedlings (KN9204, J411, BW, and Taswn-cr) grown under LN conditions (two-sided Student’s t test). Data are shown as mean ± s.d. of 3 biological replicates.