Fig. 4: The identification of chemoreceptor and peptidergic mutants that are defective in response to volatile odorants.
From: Dissecting the genetic landscape of GPCR signaling through phenotypic profiling in C. elegans
a Plate format of population chemotaxis assays used in the screen. b Violin plots of chemotaxis indices of GPCR and neuropeptide mutants to undiluted diacetyl (DA), 2, 4, 5-trimethylthiazole (TMT), isoamyl alcohol (IAA), benzaldehyde (BZ), 2,3-pentanedione (PD), 2-nonanone (NON) and 1-octanone (OCT). The strains with chemotaxis indices (>−0.5) were indicated in magenta. c The genotypes of mutants that were indicated in (b). CI refers to chemotaxis index. d Violin plots of chemotaxis indices of GPCR and neuropeptide mutants to 1:2000 diacetyl (DA), 1:1000 pyrazine (PZ), 1:2000 2, 4, 5-trimethylthiazole (TMT), 1:200 isoamyl alcohol (IAA), 1:1000 benzaldehyde (BZ), 1:10,000 2-butanone (BU), and 1:10,000 2,3-pentanedione (PD). The strains that were defective in chemotaxis to each odorant were indicated. e The genotypes of mutants that were defective in chemotaxis to various odorants as indicated in (d). The relevant genes in the strains CHS1025, CHS1135, CHS1146, CHS1173 and CHS10063 have been identified and listed in the table. CI refers to chemotaxis index. f Heatmap of the correlation between neuropeptide and neuropeptide receptor mutants in response to both odorants and V. cholerae. Red or blue color indicates the positive or negative correlation, respectively. The neuropeptide receptor mutant CHS1025 and the neuropeptide mutant CHS10063 are indicated in magenta, and their correlation is highlighted within a cyan circle.
