Fig. 5: Neuropeptide FLP-19 acts on FRPR-9 receptor to regulate AWC mediated chemotaxis.
From: Dissecting the genetic landscape of GPCR signaling through phenotypic profiling in C. elegans
a–c Chemotaxis indices to 1:10000 diluted 2,3-pentanedione (PD) of animals with indicated genotypes WT and frpr-9(yum1004) in (a), WT and flp-19(yum1005); flp-20(yum1006) double mutants in (b), and WT, frpr-9(yum1004), flp-19(yum1005), flp-20(yum1062), flp-19(yum1005); flp-20(yum1006) double, frpr-9(yum1004); flp-19(yum1005) double and frpr-9(yum1004); flp-19(yum1005) flp-20(yum1006) triple mutants in (c). p values are displayed in the plot. Two-tailed t test for (a) and (b), and one-way ANOVA, Tukey’s multiple comparison for (c). d, e Chemotaxis indices to 1:10000 diluted 2,3-pentanedione (PD) in WT, flp-19(yum1005); flp-20(yum1006) and two independent lines of transgenic flp-19(yum1005); flp-20(yum1006) expressing flp-19 genomic DNA from its endogenous promoter in (d), or expressing flp-20 genomic DNA from its endogenous promoter in (e). p values are displayed in the plot. One-way ANOVA, Tukey’s multiple comparison. f Chemotaxis indices to 1:10000 diluted 2,3-pentanedione (PD) of animals with indicated genotypes WT, frpr-9(yum1004) and two independent lines of transgenic frpr-9(yum1004) expressing frpr-9 genomic DNA from its endogenous promoter. p values are displayed in the plot. One-way ANOVA, Tukey’s multiple comparison. g, h Chemotaxis indices to 1:10000 diluted 2,3-pentanedione (PD) of animals with indicated genotypes WT (N2), flp-19(yum1005); flp-20(yum1006) and two independent lines of transgenic flp-19(yum1005); flp-20(yum1006) expressing flp-19 cDNA from mbr-1 promoter in (g), or from sra-11 promoter in (h). p values are displayed in the plot. One-way ANOVA, Tukey’s multiple comparison. i, j Chemotaxis indices to 1:10000 diluted 2,3-pentanedione (PD) of animals with indicated genotypes WT (N2), frpr-9(yum1004) and two independent lines of transgenic frpr-9(yum1004) expressing frpr-9 genomic DNA in AWC neurons from ceh-36 promoter in (i), or from both srsx-3 and str-2 promoters in (j). p values are displayed in the plot. One-way ANOVA, Tukey’s multiple comparison. In all the figure panels (a–j), data were generated from n = 3 biological replicates, and are presented as mean values +/- SEM. Source data are provided as a Source Data file.
