Fig. 7: SRX-64 is a cognate receptor for the odorant pyrazine.
From: Dissecting the genetic landscape of GPCR signaling through phenotypic profiling in C. elegans
a, b Chemotaxis indices to 1:1000 diluted pyrazine (PZ) of animals with indicated genotypes. #1 and #2 indicate two independent null alleles of srx-64 in (a). srx-64 genomic DNA was expressed from its endogenous promoter in srx-64(yum1002) in (b). p values are displayed in the plot. One-way ANOVA, Tukey’s multiple comparison. c Chemotaxis indices to different dilutions of pyrazine (PZ) in WT and srx-64(yum1002). p values are displayed in the plot. Two-tailed t test. d Chemotaxis indices of WT and srx-64(yum1002) to various diluted and undiluted odorants. p values are displayed in the plot. Two-tailed t test. e GFP is expressed from a srx-64p::srx-64::SL2::gfp polycistronic construct. mCherry expression under odr-10 promoter indicates AWA neurons. f GFP is expressed from a srx-64p::srx-64::gfp construct. mCherry under odr-10 promoter indicates AWA neurons. g Chemotaxis indices to 1:1000 diluted pyrazine (PZ) of animals with indicated genotypes. srx-64 cDNA was expressed in AWA neurons under odr-10 promoter in srx-64(yum1002). p values are displayed in the plot. One-way ANOVA, Tukey’s multiple comparison. h Heatmap (left) and average values (right) of GCaMP2 fluorescence intensity changes to 10−6 diluted pyrazine in AWA neurons of WT and srx-64(yum1002). i Chemotaxis indices to 1:1000 diluted pyrazine (PZ) of animals with indicated genotypes. srx-64 cDNA was expressed in AWB neurons under str-1 promoter in srx-64(yum1002). p values are displayed in the plot. One-way ANOVA, Tukey’s multiple comparison. j Cell surface expression of HA-SRX-64 stained with anti-HA antibody (red) and nuclei stained with DAPI (blue) in HEK293T cells. k Intracellular cAMP concentrations in response to different dilutions of pyrazine in SRX-64 (magenta) or vector (black) transfected HEK293T cells. p values are displayed in the plot. Two-tailed t test. In all figure panels (a–d, g, i, and k), data were generated from n = 3 biological replicates, and are presented as mean values +/- SEM. Source data are provided in the Source Data file.
