Fig. 5: SRGN in late-stage NPCs promotes inflammatory cytokine secretion. | Nature Communications

Fig. 5: SRGN in late-stage NPCs promotes inflammatory cytokine secretion.

From: Serglycin secreted by late-stage nucleus pulposus cells is a biomarker of intervertebral disc degeneration

Fig. 5

a–d GO and pathway analysis and GSEA of scRNA-Seq in Fibro-NPCs. e Western blotting results of four candidate inflammatory signaling pathways ((the NF-κB, AKT, Smad2/3, and ERK1/2 pathways). f Western blotting screening results of candidate subunits of the NF-κB signaling pathway. g ELISA analysis of IL-1β, TNF-α, and CCL3 in NPCs transfected with si-P65 and si-SRGN. h, j IF staining and quantitative analysis of pP65 (red) in human NPCs cultured with SRGN recombination protein (original magnification ×400, ×1000, scale bar = 50 µm, 20 µm). i–m IF staining of pP65 (red) costained with SRGN (green) and FBLN1 (purple) in WT, Srgn−/−, WT plus AF, and Srgn−/− plus AF mice (original magnification ×100, ×400, scale bar = 400 µm, 100 µm). n = 3 each group in (e) and (f); n = 5 each group in (g–m). Data are represented as the mean ± standard deviation. p values were determined by a two-tailed unpaired t test for two-group comparisons and one-way ANOVA with a post hoc Bonferroni correction for multiple groups. Source data are provided as a Source Data file.

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