Fig. 2: mtd interacts with the retromer complex and is required for retromer maintenance.
From: OXR1 maintains the retromer to delay brain aging under dietary restriction
a GO terms for the top 50 genes co-expressed with OXR1 show enrichment for endolysosomal network. Arranged by organelle and function. b, Vps35 and Vps26 levels are reduced in in heads with constitutively active pan-neuronal mtdRNAi. Quantification below. n = 5 heads per replicate per condition across minimum 3 independent experiments. Error bars represent mean value across replicates +/- SD. c Immunohistochemistry of brains from flies under constitutively active pan-neuronal mtdRNAi (bottom) show reduced Vps35 signal. Blue = DAPI, red = Vps35, green = Mtd. d Immunohistochemistry of w1118 fly brains show co-localization of Mtd and Vps35. Image below merge is magnified from boxed region in whole-brain images. e Co-immunoprecipitation in lysates from human fibroblasts transduced with GFP alone or OXR1-GFP show interaction between OXR1 and retromer proteins VPS35, VPS26A, and VPS26B. IP immunoprecipitated sample, FT sample flow-through. For all figures, *p < 0.05, **p < 0.005, ***p < 0.0005. All p values were calculated by two-sided t-test. Figure 2a was generated using BioRender (publishing license: YW266MCNQ5).
