Fig. 6: KT-NE restricts intercommunication among adipocytes in vitro and in vivo. | Nature Communications

Fig. 6: KT-NE restricts intercommunication among adipocytes in vitro and in vivo.

From: A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice

Fig. 6

a Representative digital images depicting the dynamics of lipid droplets (LDs). Experiments were repeated three times independently, with similar results. Scale bar = 30 μm, and scale bar = 12 μm in enlarged images. b Schematic overview illustrating contact-dependent and contact-independent LDs transfer between DID-labeled donor adipocytes and DIO-marked recipient adipocytes. c Schematic outline detailing the co-culturing of KT-NE-treated DID-labeled donor adipocytes (red) and untreated DIO-labeled recipient adipocytes (green). d Representative fluorescence images demonstrating non-contact-dependent LDs transfer and (e) contact-dependent LDs transfer between DID-labeled donor adipocytes (KT-NE treated, red) and DIO-marked recipient (untreated) adipocytes (green). Extracellular/intracellular red dots represent DID-marked membrane-encapsulated small lipid droplets secreted by DID-marked donor adipocytes (red), highlighted with red triangles. Internalized and accumulated DID-marked membrane-encapsulated small lipid droplets into DIO-recipient (especially into the larger lipid droplets) were marked with orange triangles. Experiments were repeated three times independently, with similar results. Scale bar = 30 μm, and scale bar = 6 μm in enlarged images. f Diagrammatic sketch of co-culturing untreated DID-labeled donor adipocytes (red) and KT-NE-treated DIO-labeled recipient adipocytes (green). g Representative fluorescence images demonstrating non-contact-dependent LDs transfer and (h) contact-dependent LDs transfer between DID-labeled donor adipocytes (untreated, red) and DIO-marked recipient (KT-NE treated) adipocytes (green). Experiments were repeated three times independently, with similar results. Scale bar = 30 μm, and scale bar = 6 μm in enlarged images. i Sketch map illustrating DIR-labeled mixed lipids biodistribution in mice with obesity (n = 5 biologically independent animals) pre-treated with indicated NEs for 1 week. j Biodistribution and (k) relative fluorescence transmission (or diffusion) distance/area of DIR-labeled mixed lipids in subcutaneous adipose tissue (SAT), after DIR-labeled mixed lipids injected (s.c.) into the hosts for 48 h. n = 10 SAT from five biologically independent animals. Source data are provided as a Source Data file. Statistical significance was evaluated by an unpaired two-tailed t-test. All data was expressed as mean ± SD, ns no significance.

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