Fig. 1: Phenotypic analysis of LBL-induced leaf senescence in the wild-type TL1 and Gmcry mutants.

a Experimental scheme for LBL treatment. For a pair of unifoliate leaves, one was covered with two layers of yellow filter to imitate the LBL condition, and the other one was covered with two layers of transparent filters as the control. White and yellow arrows indicate the transparent and yellow filters, respectively. b Leaf senescence phenotypes of wild-type TL1 cultivar induced by LBL treatment. Seedlings were de-etiolated under continuous white light for 10 days, then a pair of unifoliate leaves were treated with different light regimes (LBL or WL) for 14 days as in (a). Scale bar, 5 cm. c Chlorophyll content in the leaves as in (b). Values are means ± SD (n = 5 biological replicates). d Relative transcript levels of senescence-associated genes GmSAG12, GmSAG13, and GmSAG113 in the leaves as in (b). Values are means ± SD (n = 3 biological replicates). The GmActin gene was used as the internal control. e Leaf senescence phenotypes of indicated lines treated by WL and LBL as in (b). Scale bar, 3 cm. f Chlorophyll content in the leaves as in (e). The percentage decrease in chlorophyll content under WL compared to LBL is indicated by the values above the respective p values. Values are means ± SD (n = 5 biological replicates). g Relative transcript levels of senescence-associated genes in the indicated lines in response to LBL treatment as in (b). The unifoliate leaves were collected for RT-qPCR analysis. Values are means ± SD (n = 3 biological replicates). The GmActin gene was used as the internal control. All above P values were calculated by unpaired two-tailed t-test. Source data are provided as a Source Data file.