Fig. 1: TFIP11 interacts with the BLM complex.

A Tandem affinity purification (TAP) procedure. B, C HEK293T cells stably expressing either vector control or SFB-tagged TFIP11 were harvested and lysed with NETN buffer for 30 min on ice. Crude lysates were centrifuged at 4 °C, 14,000 × g for 8 min and the resulting supernatants were incubated with streptavidin beads at 4 °C for 2 h. After three washes, immunocomplexes were eluted with NETN buffer containing 1 mg/ml biotin. Elutes were then incubated with S-protein beads at 4 °C for 2 h. The final eluates were resolved by SDS-PAGE, stained with Coomassie blue (B) or subjected to mass spectrometric analysis (C). D, E Association of endogenous TFIP11 with the BLM complex. HeLa cells were mock treated or treated with 4 mM HU for 3 h. The cells were then lysed with NETN buffer in the presence or absence of Benzonase. The resulting cell lysates were incubated with protein A agarose beads conjugated to either anti-TFIP11 antibodies (D) or anti-BLM antibodies (E), and then subjected to Western blotting. Source data are provided as a Source Data file.