Fig. 6: Performance of Cir-reporters in a standard CRISPR/Cas12a biosensing system (Methods 13, 14). | Nature Communications

Fig. 6: Performance of Cir-reporters in a standard CRISPR/Cas12a biosensing system (Methods 13, 14).

From: Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing

Fig. 6

A Unquenching schematics of Cir-reporters; B Comparison of the fluorescence signals of a Cir-reporter and linearized Cir-reporter (L-Cir-reporter) (18nt dsDNA with 3nt ssDNA) (n = 3 independent reactions); C Background signals of Cir-reporters with different linker lengths (18nt dsDNA) (n = 3 independent reactions). L-x denotes the linker length of x nt; D Investigation of the Cir-reporter linker length in a standard CRISPR/Cas12a biosensing system (18nt dsDNA, 100pM target DNA) (n = 3 independent reactions); E Comparison of the detection limits of standard CRISPR/Cas12a biosensors with Cir-reporters (18nt dsDNA with 3nt ssDNA) and with linear ssDNA reporters (TTATT) with identical fluorophore-quencher pairs (n = 3 independent reactions). (Two tails student T test, Error bars represent mean ± SD, *P < 0.05, **P < 0.005, ***P < 0.001, a.u = arbitrary units).

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