Fig. 4: OCT-1 and OC proteins promote HSV-1 replication and/or reactivation.
a The indicated cells were infected with KOS (MOI = 0.3) for 48 h before virus titration. b Neuro-2a cells were transfected with 80 nM siRNA for 40 h, then infected with KOS (MOI = 0.2) for 48 h before virus titration. c Neuro-2a cells were transfected with 200 ng/ml plasmid for 24 h, then infected with WT KOS or ICP0-null virus (MOI = 0.2) for 48 h (left) or the indicated times (right) before virus titration. d HFF cells were transduced with lentivirus for 72 h, then infected with KOS (MOI = 0.05) for 48 h before virus titration. e Primary TG neurons were transduced with lentivirus for 4 days, then infected with 64-GFP (MOI = 0.1) for 48 h before immunofluorescence analysis of TUJ1 and visualization of GFP (green) and mCherry (red) expression. The experiment was repeated once with similar results. f Primary TG neurons were transduced with lentivirus for 4 days, then infected with KOS (MOI = 0.1) for the indicated times before titration of the virus in the supernatants. g Differentiated human neurons were transduced with lentivirus for 10 days, then infected with KOS (MOI = 1) for 48 hpi before virus titration. The data are from 4 biologically independent experiments with 4 replicates per experiment. Each point represents the mean from one experiment. h TG neurons were transduced with lentivirus and supernatants were assayed for infectious virus positivity at the indicated days after addition of DMSO (left), TSA (0.2 μM) (left) or LY294002 (20 μM) (right). n = 3 (a, c, d), 4 (b), 6 (f) or 16 (g) biologically independent samples. Sample sizes for (h) are labeled on the figure. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparisons tests (a, b, d, f and left panel of c), two-sided unpaired t tests (right panel of c), two-way ANOVA, matched by experimental batch, with Ĺ Ădák’s multiple comparisons tests (g) or Log-rank (Mantel-Cox) tests (h) and are presented as the mean ± s.d. Source data are provided as a Source Data file.
