Fig. 2: Probing sugar donor selectivity of SenB.

a, i) SenB catalyzes the synthesis of selenosugars using SeP; ii) Derivatization of selenosugars using mBBr. b Chemical structures of sugar donors. c Kd values of SenB for binding of different sugar donors. d HPLC-UV/DAD analysis at 391 nm of the mBBr derivatives of the SenB reaction products using different sugar donors.