Fig. 5: Catalytic mechanism of SenB. | Nature Communications

Fig. 5: Catalytic mechanism of SenB.

From: Substrate binding and catalytic mechanism of the Se-glycosyltransferase SenB in the biosynthesis of selenoneine

Fig. 5

a Catalytic activity center and critical catalytic residues of SenB. Hydrogen bonds are represented by black dashed lines, water bridges by green dashed lines, and water molecules by red spheres. b The pKa value of the selenol group (-SeH) of SeP. c Relative catalytic activities of the SenB mutants using UDP-Glc as the sugar donor. Data are presented as mean values ± SD (n = 3 independent experiments). Source data are provided as a Source Data file. d LC-MS detection of the selenophosphosugar intermediates from catalysis by the K158 mutants. e The K158A mutant halts the reaction of the Se-P bond cleavage. f Proposed reaction mechanism for the Se-glycosylation of SenB.

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