Fig. 3: GRB2 promotes reverse fork stability by inhibiting RAD51 ATPase activity. | Nature Communications

Fig. 3: GRB2 promotes reverse fork stability by inhibiting RAD51 ATPase activity.

From: GRB2 stabilizes RAD51 at reversed replication forks suppressing genomic instability and innate immunity against cancer

Fig. 3

a RAD51 interacts with the SH2 ___domain of GRB2. MST binding isotherms measuring binding affinity of RAD51 with wild type GRB2, SH2 ___domain and indicated GRB2 mutant. The binding affinities (Kds) mean values with ±SD are shown below, n = 3. b ATPase activity assay showing GRB2 dose-dependent inhibition of RAD51 ATP-hydrolysis with ±SD, n = 3. c Representative gel-image showing GRB2 dose dependent RAD51 strand-stabilization to dsDNA with 5′ overhang. d Normalized quantitation of RAD51 strand exchange and stability induced by GRB2 with ±SD, n = 3. e Representative PLA images of RAD51 foci formation with ssDNA. f Quantitation of nascent DNA associated RAD51 collected from >350, n = 3. Scale bar 10 μm. The significance was analyzed by two-sided Student’s t test. ****P ≤ 0.0001; NS not significant. For a–d and f source data are provided as a Source Data file.

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