Fig. 2: Atg5 deletion changes hematopoietic progenitor cell function and HSC differentiation in the fetal liver.

a, b Methylcellulose culture data showing the number of CFU-Cs and number of each hematopoietic colony type in the E12.5 fetal liver (indicated by color bars) per embryo equivalent (ee) or per 1000 input cells of control and KO. Error bars represent mean ± SEM. n = 4 biologically independent embryos, *p < 0.05, **p = 0.0016. c–e Flow cytometric analysis showing the percentage of Lin⁻, Lin⁻Sca1⁺Mac1^low (LSM) and CD201⁺LSM (HSC) in E12.5 fetal liver cells. n = 7 biologically independent embryos, **p = 0.006. Circle = control (Ctr), inverted square = KO. f The number of CD45⁺ hematopoietic cells from E12.5 fetal liver (FL) HSCs was unchanged. n = 4 biologically independent experiments. g Atg5 deletion resulted in the reduced hematopoietic differentiation capacity of HSC in the E12.5 FL HSC. n = 4 biologically independent experiments. *p = 0.02. h The lineage output of HSC in E12.5 KO FL compared with control group. Error bars represent mean ± SEM. n = 4 biologically independent experiments. *p < 0.05. i–k The number of erythroid, myeloid and lymphoid cells from the same input HSCs (10 cells) in E12.5 KO FL compared with control group. n = 4 biologically independent experiments, *p < 0.05. Circles = control (Ctr), inverted squares = KO. Statistical significance was determined by one side Student’s t-test.