Fig. 6: CWC15 promotes 20 S proteasome activity.
From: The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis
a The protein interactions analyzed by BiFC. Paired cYFP- and nYFP-fusion proteins were co-expressed in tobacco leaves. Green color indicates the BiFC signal detected by a confocal microscopy at 48 h after infiltration. Scale bar = 10 μm. Three independent experiments yielded consistent results. b The association of CWC15 with PAG1 and PAB1 detected by Co-IP. PAG1−2HA, PAB1-2HA and GFP-2HA were co-expressed with MYC-CWC15 in leaves of N. benthamiana, respectively. IPs were performed using anti-MYC antibodies. PAG1-2HA, PAB1-2HA, GFP-2HA and MYC-CWC15 were detected by western blot. Two independent experiments yielded consistent results. c Co-IP between CWC15 and PAG1 in plants containing the p35S::2HA-CWC15 and pPAG1::gPAG1-FM transgenes. 2HA-CWC15 and PAG1-FM were detected by western blot. Numbers on top of the pictures indicate plants used: 1, 2HA-CWC15/gPAG1-FM; 2, Col-0; 3, gPAG1-FM. Two independent experiments yielded consistent results. d The enrichment of SE in the PAG1 complex with or without MG132 treatment. IP was performed using anti-FLAG antibodies. SE and PAG1-FM were detected by western blot using anti-SE and anti-FLAG, respectively. Numbers on top of the pictures indicate plants used: 1, Col-0; 2, gPAG1-FM, 3, amiRCWC15/gPAG1-FM. The enriched SE protein compared with IPed PAG1-FM in amiRCWC15 was normalized to that of Col-0, which was assigned a value of 1. Two independent experiments yielded consistent results. Source data are provided as a Source Data file.
