Fig. 6: Single-cell sequencing characterized changes in T cell molecules and TCR.

a UMAP graph displaying T cell subpopulations and clustering. b Box plot showing difference in T cell toxicity scores between both groups. c Box plot showing difference in Treg activation gene set scoring between both groups. Minima: Lower limit of the whisker; Maxima: Upper limit of the whisker; Centre: Median line inside the box; The upper and lower box bounds represent the 25% and 75% percentile of data for (b) and (c). Two tailed Wilcoxon rank sum test. d Violin diagram showing the expression level of Treg activity inhibitory gene in the control and BA.2-BTI-6m groups. P values are calculated by the two tailed Wilcoxon rank sum test, ****: p < 0.0001, ns: p > 0.05. e The heat map shows the biological process of Treg differential gene enrichment. f UMAP diagram showing T cells with TCR information. g Bar chart indicating proportion of TCR detected in T cell subsets. h Bar chart showing TCR clonal proportion of a single sample. i Bar chart showing difference in TCR diversity between both groups. P values are calculated by the two tailed Wilcoxon rank sum test, Control (n = 4), BA.2-BTI-6m (n = 5). j Stacked bar chart displaying proportion of clonal T cell subpopulations. k The circular plot shows the proportion of TCR with different frequencies in the control and BA.2-BTI-6m groups. l Bar chart showing top 10 TCR CDR3 sequences specifically amplified in control or BA.2-BTI-6m groups. m Venn chart showing intersection of TCR in control group samples. n Venn chart showing intersection of TCR of samples from BA.2-BTI-6m group. o The violin diagram shows the differential genes upregulated and downregulated by top 10 in CD8 TEM. p Functional enrichment analysis of upregulated and downregulated DEGs by CD8 TEM.