Fig. 7: Single-cell sequencing characterized changes in B cell molecules and B cell receptor (BCR).

a UMAP graph displaying subpopulations and clustering of B cells. b UMAP map showing expression of marker genes in B cell subpopulation, including naïve B cells (MS4A1⁺ IGHD⁺), memory B cells (MS4A1⁺ CD27⁺), intermediate B cells (IGHD⁺ CD27⁺), and germinal center B cells (MS4A1⁺ NEIL1⁺). c Box plot showing differences in antigen processing and presentation as well as B cell activation scores of B cell subpopulations between control and BA.2-BTI-6m groups. Minima: Lower limit of the whisker; Maxima: Upper limit of the whisker; Centre: Median line inside the box; The upper and lower box bounds represent the 25% and 75% percentile of data. Two tailed Wilcoxon rank sum test. d, e The violin diagram shows the differential genes upregulated and downregulated by top 10 in naïve B cells. f Functional enrichment analysis of differential genes by naïve B cells. g Correlation between antigen presentation, interferon and B cell activation module scoring and neutralizing activity of different virus strains. ***p < 0.001, **p < 0.01, *p < 0.05. h UMAP diagram showing B cells with BCR information. i Bar chart showing proportion of BCR detected in B cell subsets. j Bar chart indicating BCR clonal proportion of a single sample. k The circular plot shows the proportion of BCR with different frequencies in the control and BA.2-BTI-6m groups. l Stacked bar chart displaying proportion of clonal subtypes in B cell subpopulations. m Bar chart showing most-amplified specific BCR CDR3 sequence in control and BA.2-BTI-6m groups. Pink: control, green: BA.2-BTI-6m.