Fig. 4: Ferroptotic iron overload triggers ODC1 expression.
From: Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer
a Heatmaps exhibiting the expression levels of a series of polyamine metabolism-related genes in A549 or HT1080 cells treated with DMSO or RSL3 (A549: 2 μM; HT1080: 0.2 μM) for 4 h, determined by RNA-seq, n = 4. b mRNA levels of ODC1 in A549 or HT1080 cells treated with DMSO or RSL3 (A549: 0.5 μM; HT1080: 0.05 μM) combined with or without DFO or Fer-1 for 24 h, determined by qPCR. c Protein levels of ODC1 in A549 or HT1080 cells treated with DMSO or RSL3 (A549: 0.5 μM; HT1080: 0.05 μM) combined with or without DFO or Fer-1 for 24 h, determined by western blotting. d Quantification of 15N4 abundance in the indicated polyamines in DMSO or RSL3 (0.5 μM for 24 h) treated A549 using LC-MS. e Relative abundance of polyamines in A549 and HT1080 cells treated with DMSO or RSL3 (A549: 0.5 μM; HT1080: 0.05 μM) for 24 h, determined by LC-MS. f Confocal microscope images of FerroOrange-stained A549 or HT1080 cells treated with RSL3 (A549: 2 μM; HT1080: 0.25 μM) combined with or without DFO (100 μM) or Fer-1 (10 μM) for 3 h (scale bars, 100 μm). g, h mRNA (g) and protein (h) levels of ODC1 in A549 or HT1080 cells treated with FAC as indicated for 24 h. i Relative abundance of polyamines in A549 and HT1080 cells treated with FAC for 24 h, determined by LC-MS. Data are presented as the mean ± SD, n = 3 independent experiments unless otherwise stated. Unpaired two-tailed Student’s t tests are used. Source data are provided as a Source Data file.
