Fig. 6: Loss of GAS41 promotes tumor suppression, at least partially through ferroptosis in vivo.

a Weight quantification of tumors derived from sgNC and sgGAS41 H460 cells as indicated. b Western blot of GAS41, SLC7A11, GCLC, and NRF2 protein levels of tumor tissues in (a). c Measurement of GSH concentration of tumor tissues in (a). d Assessment of lipid peroxidation by flow cytometry after C11-BODIPY staining of tumor tissues in (a). e RT-qPCR analysis of PTGS2 mRNA expression levels of tumor tissues in (a). f, g Image (f) and weight quantification (g) of xenograft tumors derived from sgNC, sgGAS41 A549, or sgGAS41 A549 cells administrated with Ferr-1 as indicated. h, i Assessment of lipid peroxidation (h) and statistical bar graph (i) by flow cytometry after C11-BODIPY staining of tumor tissues in (f). j Box plot of SLC7A11 and GCLC mRNA expression of YEATS4-unaltered (NFE2L2, KEAP1, YEATS4 WT, n = 328) or -amplified (n = 13) patients from TCGA-LSCC (lung squamous cell carcinoma) analyzed by cBioPortal. The median value is shown in the box. The whiskers indicate the value of minima and maxima, and the box bounds indicate the value of the first quartile or third quartile. k Kaplan–Meier plots of TCGA-LUAD (lung adenocarcinoma) patients stratified by unsupervised clustering on YEATS4, SLC7A11, and GCLC expression. The blue line has lower SLC7A11, lower GCLC, and lower YEATS4 expression (n = 239), while the red line has higher SLC7A11, higher GCLC, and higher YEATS4 expression (n = 239). For c, d, and i data are mean ± SD of n = 4, for e data are mean ± SD of n = 6 independent biological repeats. For a, g data are mean ± SEM of n = 6 independent tumor samples. p values were calculated using unpaired, two-tailed Student’s t test. Source data are provided as a Source Data file.