Fig. 1: Kernel phenotypes of B73 and fka1-1 harvested in Sanya.
From: An ARF gene mutation creates flint kernel architecture in dent maize
a The kernel phenotype of B73 and fka1-1. Scale bar, 1 cm. b Longitudinal-sections of mature B73 and fka1-1 kernels. The angles of the dent on B73 and fka1-1 kernel crowns are a measure of the degree of the dent. Scale bar, 2 mm. c Statistical measurements of kernel crown collapse in B73 and fka1-1. Schematic diagrams showing area measured are indicated in (b). Data are mean ± s.d. (n = 50 kernels from 20 ears). d Ratio of vitreous endosperm (VE) area to the total endosperm. Data are mean ± s.d. (n = 50 kernels from 20 ears). e Breakage rate of kernels. Data are mean ± s.d. (n = 20 ears). f Longitudinal-sections of B73 and fka1-1 developing kernels at 15, 20, 25, 30 and 35 days after pollination (DAP). The red dotted line marks the area measured for pericarp length. Black arrows indicate area of incomplete grain filling. Scale bar, 2 mm. g Pericarp length of B73 and fka1-1 kernels at different developmental time points. The schematic diagrams show measurements in (f). Data are mean ± s.d. (n = 88 kernels from 11 ears). h Dry weight of 100 mature kernels ofB73 and fka1-1. Data are mean ± s.d. (n = 20 ears). i Light microscopy and TEM of sections of kernel crown regions of B73 and fka1-1 at 30 DAP. Scale bars in semi-thin sections, 100 μm; scale bars in TEM, 20 μm. Al, aleurone; En, endosperm; Em, embryo; P, protein body; S, starch granule; SE, starchy endosperm; VE, vitreous endosperm. Two-tailed Student’s t-tests were used to determine P-values shown in the (c–e, g, h).
