Fig. 1: Schematic illustration of CAT-S.
From: Bioorthogonal photocatalytic proximity labeling in primary living samples
CAT-S is an in situ subcellular proteomics strategy that utilizes the cell-permeable Ir-based photocatalyst to target mitochondria in living cells from primary tissue or blood samples, which can catalyze the blue light-triggered bioorthogonal decaging reaction of the p-azidobenzyl (PAB) group to generate reactive thioQM probe for in situ proximity labeling of the mitochondrial proteome. No genetic engineering is required for CAT-S, rendering it widely applicable for various primary and living samples. Created with BioRender.com (agreement number JH26IJNP4R).
