Fig. 4: Gadd45g deficiency-induced MPN is transplantable and reintroduction of Gadd45g prominently prolongs the survival of mice with MPN.

a–j One million whole BM cells from the Ctrl mice or moribund mice with MPN (CD45.2) were transplanted into lethally irradiated recipients (CD45.1) (5 recipients per group). The hematological parameters were examined when the recipient mice required euthanasia because of moribund conditions. Kaplan-Meier survival curves of the recipient mice (log-rank test) (a). Percentages of donor-derived overall (CD45.2⁺) and those of myeloid (Mac-1⁺), B (B220⁺), and T (CD3⁺) cells in CD45.2⁺ PB of recipients (b). Representative images of spleens from recipient mice (left) and spleen weights of recipient mice (right) (c). H&E staining of femur (top) and spleen (bottom) sections from recipient mice. Bars represent 100 μm. At least three independent biological replicates were performed (d). Absolute number of Gr-1⁺ (e), Mac-1⁺ (f), Gr-1⁺Mac-1⁺ (g), B220⁺ (h), CD3⁺ (i) and Ter119⁺ (j) cells in the BM (left) and spleen (right) of recipient mice. (k) Lin^-c-kit⁺ HSPCs isolated from moribund mice with MPN were transfected with Dox-inducible Gadd45g lentiviral vector and then transplanted into lethally irradiated recipient mice (10⁴ cells per mouse). Dox (1 mg/mL) was added to drinking water 7 days after transplantation for 1 week to induce Gadd45g expression. Kaplan-Meier survival curves of recipient mice in different groups were shown (n = 6 mice per group, log-rank test). For (b, c, e–j): Data are shown as means ± SD. Two-tailed Student’s t test.