Fig. 4: A conserved yet environmentally tuned condensation response is adaptive across species with differing temperature optima for growth.
From: An adaptive biomolecular condensation response is conserved across environmentally divergent species
a DLS temperature ramp experiments of 15 µM Pab1 from each species, all at pH 6.4. b Correlation of Tcondense (°C) with optimum growth temperature (circles) or maximum growth temperature (triangles). Error bars for Tcondense (°C) represent the standard deviation of the mean of at least three replicates. Error bars for the optimum and maximum one standard deviation of the parameter estimates from the fit of the cardinal temperature model with inflection. Error bars for S. cerevisiae calculated from results are from n = 4, n = 5, and n = 6 for MV → I, WT, and MV → A, respectively. Error bars for S. kudriavzevii calculated from results are from n = 3, n = 4, and n = 3 for MV → I, WT, and MV → A, respectively. Error bars for K. marxianus calculated from results are from n = 4, n = 5, and n = 4 for MV → I, WT, and MV → A, respectively. c DLS temperature ramp experiments of 15 µM Pab1 mutants from each species, all at pH 6.4. Twelve mutations were made for each mutant from each species. Curves for S. cerevisiae mutants and wildtype are from Riback & Katanski et al. 2017. d Comparison of Tcondense (°C) values calculated for each Pab1 protein. Tcondense (°C) was calculated for S. cerevisiae mutants and wildtype from the curves contained in Riback & Katanski et al. 2017. e Spot assays of S. cerevisiae and S. kudriavzevii strains containing mutations in the P ___domain. Plates were incubated at 23, 32, and 40 °C for four days (96 hours), and then shifted to 23 °C and grown for two days (48 hours). Columns are 10-fold dilutions. Source data are provided as a Source Data file.
