Fig. 2: EGLN1 positively regulates cellular antiviral immune responses depending on its enzymatic activity.
a qPCR analysis of IFN-β mRNA in EGLN1 +/+ and EGLN1 −/− H1299 cells infected with SeV, VSV or HSV-1 for 0, 4 and 8 h. b EGLN1+/+ and EGLN1 −/− H1299 cells were infected without (UI) or with VSV-GFP virus for 12 h, and viral infectivity was detected by fluorescence microscopy (top panels) or flow cytometry analysis (bottom panels). c qPCR analysis of IFN-β mRNA in EGLN1+/+ and EGLN1−/− THP-1 cells infected with SeV, VSV or HSV-1 for 0, 4 or 8 h. d qPCR analysis of IFN-β mRNA in EGLN1+/+ and EGLN1−/− RCC4 cells infected without (UI) or with VSV or HSV-1 for 8 h. e qPCR analysis of IFN-β mRNA in EGLN1+/+ and EGLN1−/− 786-O cells infected without (UI) or with VSV or HSV-1 for 8 h. f qPCR analysis of IFN-β mRNA in HEK293T cells transfected with the HA empty vector (EV), the plasmid expressing HA-EGLN1 (WT) or the plasmid expressing the enzymatically inactive mutant of EGLN1 (H313A) for 24 h, followed by infection without (UI) or with SeV for 8 h. g qPCR analysis of IFN-β in H1299 cells treated with DMSO (vehicle control) or FG4592 (20 μM) for 6 h, followed by infected without (UI) or with HSV-1 for 8 h, or transfected without (UT) or with poly (I:C) for 8 h, or transfected without (UT) or with poly (dA:dT) for 8 h. h H1299 cells were treated with DMSO (vehicle control) or FG4592 (20 μM) for 6 h, followed by infection without (UI) or with VSV-GFP virus for 12 h, and viral infectivity was detected by fluorescence microscopy analysis. BR, bright field. EGLN1−/− H1299 cells are clonal. Data in (a, c–g) are presented as mean ± S.D., two-way ANOVA; n = 3 biological independent experiments. Data in (b, h) are presented as mean ± S.D., two-tailed Student’s t test; n = 3 biological independent experiments. See also Supplementary Figs. 2–5. Source data are provided as a Source data file.
