Fig. 2: A single vector system for dual inhibition in CBE.
From: Enhancing genome editing in hPSCs through dual inhibition of DNA damage response and repair pathways
A Scheme of AncBE4stem construction, created with BioRender.com. B–D C to T (B), C to G (C) conversion efficiency, and total relative base conversion (D) (n = 3 except the designated replicates, n.d. for not detected data) of AncBE4max and AncBE4stem at the indicated target sites. E Off-target editing of CCR5-3 targeted sites (OT1, OT2, OT3 and OT4) and HEK2 targeted sites (OT1, OT2 and OT3) with AncBE4max and AncBEstem at the indicated target sites (n = 3). F Deletion more than 100 base pair long at RNF2 target treated with AncBE4max and AncBE4stem analyzed by Nanopore long-read sequencing (n = 3). G A jitter plot of the DNA C to T substitution and RNA C to U from whole-exome sequencing data and transcriptome data of AncBE4max and AncBE4stem treated cells with HEK3 targeting gRNA (n = 1). n always represents the biologically independent samples if not else described. Bars represent mean values, and error bars represent the S.D. of independent biological replicates. Detailed information on statistical analysis is listed in the “Statistical analysis” section. The source data of B-F are provided in the Source Data file.
