Fig. 6: Impact of hepatic RRBP1 deletion on ER-mitochondria interactions and mitochondria structure and function.

A, B Representative TEM images of livers from wild-type (WT) and RRBP1 deficient (RRBP1-KO) mice in fasted state. Scale bars: 500 nm. C Quantification of mitochondria surface covered by ER between 25-80 nm distance. n = 208 for Wt and n = 311 RRBP1-KO. D–F Quantification of mitochondria area (D), roundness (E) and aspect ratio (AR) (F), n = 208 for Wt and n = 308 RRBP1-KO. G ¹⁴C-palmitic acid-driven fatty acid oxidation in hepatocytes from wild-type control (n = 8) and RRBP1 KO (n = 8). H, I Oleate and palmitate driven lipid loading assay and quantification analysis in primary hepatocytes from wild type (Wt) and RRBP1 deficient cells (n = 107 cells for Wt, n = 133 cells for RRBP1 KO). Lipid droplets were stained with BODIPY (green) and RRBP1 was stained in red. Scale bars: 10 µm. J, K Oleate driven lipid loading assay in primary hepatocytes infected with adenovirus expressing GFP (ad-GFP) and adenovirus expressing RRBP1-GFP (ad-GFP-RRBP1), (GFP n = 6; RRBP1 NT n = 5, GFP Oleate n = 6, RRBP1 Oleate, n = 7). Scale bars: 5 µm. For the bar graphs data are shown as mean ± s.e.m.; For C, E, F, G, I, K, two-tailed unpaired t-test ****p < 0.0001, **p < 0.003.