Fig. 3: Generation of hHOs.

a The overall strategy to generate hHOs. b The typical morphology of hHOs at different ages. Scale bar: 100 µm. c The significant markers in the developmental process of the hHO. d Staining images of day-30 hHOs. Upper: Lager-scale image of the whole hHO. Scale bar: 100 µm. Lower: Zoom-in greyscale view of the white box in the upper figures. Scale bar: 40 µm. e The hippocampal PAX6⁺ and HOPX⁺ progenitors in day-60 hHOs. Scale bar: (left) 100 µm and (right, zoom-in greyscale view of the white box in the left figures) 40 µm. f The hippocampal PROX1⁺ cells in day-60 hHO and day-90 hHO. Scale bar: 150 µm. g qPCR for genes expressed in day-117 hHOs, day-71 hHOs versus day-46 hHOs (n = 3, three independent measurements using three independent samples, one-tailed t-test). h, qPCR for genes expressed in day-45 hHOs versus day-45 DMT organoids (n = 3, 3 independent measurements using three samples from different organoids, one-tailed t-test). Images in d–f are representative of n = 3 independent experiments. Exact sample size and values for h and g are provided in Source Data. Source data are provided as Source data files. hHO human hippocampal organoid, DMT dorsomedial telencephalon.