Fig. 2: Binding kinetics of VHHs to purified toxins. | Nature Communications

Fig. 2: Binding kinetics of VHHs to purified toxins.

From: In vivo neutralization of coral snake venoms with an oligoclonal nanobody mixture in a murine challenge model

Fig. 2

Biotinylated toxins captured on streptavidin biosensors were dipped in decreasing concentrations of each of the VHHs, followed by dissociation in kinetics buffer. Binding data were fitted using a 1:1 model. The colors represent the different VHH concentrations: black is 200 nM, pink is 67 nM, green is 22 nM, dark purple is 7.4 nM, light purple is 2.5 nM, and cyan is 0.8 nM. A–C Anti-PLA2 VHHs binding PLA2N. D–F Anti-αNTx VHHs binding αNTx DH. Source data are provided as a Source Data file.

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