Fig. 3: Ahed deletion causes severely anaemic and embryonic demise.

a Genotyping results of the pups and embryos obtained from Ahed^+/−; Vav1-cre and Ahed^fl/fl pairs at the indicated points. b Embryos recovered at E12.5. Asterisks indicate the Ahed cKO (Vav1-cre Ahed^fl/fl) embryos. Scale bars, 5 mm. c Embryos recovered at E16.5 (left). Arrows indicate Ahed cKO embryos. Scale bar, 5 mm. The embryo in the dotted square exhibits subcutaneous oedema (arrowhead, right). d E14.5 foetal liver cells were stained with an isotype control IgG (dashed line) or an anti-TER119 Ab (solid line) and analysed by flow cytometry. Representative data for the indicated genotypes are shown. Absolute numbers of TER119⁺ erythroid cells in E14.5 foetal liver were calculated from the flow cytometric data. Horizontal lines indicate median values. e Graph showing the proportion of E14.5 foetal liver erythroid cells in each developmental stage. ProE, proerythroblasts; B, basophilic cells; Poly, polychromatic cells; A, acidophilic cells; R, denucleated red blood cells. f Flow cytometry plots of E14.5 foetal liver erythrocytes from Vav1-cre Ahed^fl/+ (control) and Vav1-cre Ahed^fl/fl (cKO) mice to evaluate erythroid differentiation using Ter119 and CD71. Each subset group was defined as follows; S0, TER119⁻CD71⁻; S1, TER119⁻CD71⁺; S2, TER119^LoCD71⁺; S3, TER119⁺CD71⁺; S4, TER119⁻CD71⁺. Numbers indicate the percentage of each fraction. Results shown are representative of three independent experiments. Data are presented as mean ± s.d. Statistical significance in a was determined by one-sided Chi-square test, *against expectations of Mendelian segregation, in d, f by one-way ANOVA with Brown-Forsythe test, and in e by two-sided unpaired Student’s t-test. *p < 0.05; ****p < 0.0001; NS, not significant. Source data are provided as a Source Data file.